In effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of tridax procumbens flowers extract against e. The extracts of tridax procumbens have been reported to have various pharmacological effects, antimicrobial activity, wound healing property immunomodulatoryand effect on experimental animals. Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. They represent rich source of antimicrobial agents 2. Comparative study of antimicrobial activity of tridax. Besides, antioxidant activity of ethyl acetate extract ic. Chitra pai 1, ujjwala kulkarni 2, manjusha borde 2, sowmya murali 3, p.
The leaf juice possesses antiseptic, insecticidal and parasiticidal properties and is used. The methanolic extract of flowers of tridax procumbens was evaluated for their mic against e. List of various pharmacological activity of parts of plant tridax procumbenc linn. Jun 09, 2015 herbal products derived from hemidesmus indicus l. Assessment of antibacterial and antifungal activities of silver nanoparticles obtained from the callus extracts stem and leaf of tridax procumbens l. Pdf antibacterial activity of tridax procumbens with special. Plants are known to provide biologically active molecules with reduced or no toxicity. Fehlings solution, tridax procumbens, cu2o nanoparticles, polyaniline, li 1. Methanol extract of tridax procumbens showed maximum antifungal activity. Pdf antibacterial activity of tridax procumbens linn. Antibacterial activity of methanolic extract of tridax procumbens l. In this study, we evaluated the potential use of t. Antibacterial evaluation of aqueous and methanol extracts. Ethyl acetate extract of this plant was separated to different fractions by column chromatography cc using chloroform and methanol as.
The extracts were stored in desiccators for preliminary phytochemical analysis and further testing of antimicrobial activity. The present work is concerned with exploring antimicrobial activity of chloroform extract and aqueous extract. Introduction in the fields of nanoscience and nanotechnology, the largest activity has been focused on the synthesis of new nanoparticles with different sizes and new shapes. Many reports has been given for the same pathogenic organisms28. Antioxidant, antidiabetic, antimicrobial and hemolytic activity of tridax procumbens r. Antihyperuricemia, antioxidant, and antibacterial activities of tridax. Evaluation of phytochemical and antioxidant activity of.
January antimicrobial activity of tridax procumbens. In the antibacterial activity assay, different extracts of t. We have synthesized silver nanoparticles by adding the 50ml of 1mm silver nitrate solution in to the plant extract and characterized by uvvis absorption spectroscopy, and sem techniques. The antimicrobial activity of tridax procumbens performed by using agarwell diffusion method showed a result showcasing an effective limit when as opposed to pseudomonas vulgarisfor ethyl alcohol being used as solvent for extract. The present study concluded that tridax procumbens can be used as a source for green synthesis of agnps which possesses potent antibacterial and antioxidant activities. The results are compared with standard antibiotic streptomycin. Herbs are natural remedies for the disease with higher safety profile and efficacy. Dada2 1department of microbiology, adekunle ajasin university, akungbaakoko ondo state, nigeria.
Silver nanoparticles agnps were synthesized by reducing 2 mm solution of agno3 using leaf extract of tridax procumbens. Antimicrobial activity is mostly investigated by disc diffusion method and agar well diffusion method 6. The mean of three replicates of zone of inhibition mm around well with tridax procumbens mediated fe3o4 nanoparticles is presented in the table 1. Antimicrobial activity preliminary phytochemical screening of ethanolic culturemedia and inoculums. In this present study focused on bacterial cellulose implementation with this plant. Various extracts on waterborne bacterial pathogens uzma bathool, naziya habeeb m. The antioxidant activity of synthesized agnps and aqueous extract of tridax procumbens leaves was determined using dpph free radical scavenging, metal chelating, and. Tridax procumbens using 500ml of 80% ethanol in a soxhlet extractor for 48 hours. Tridax procumbens have been used as indigenous medicine for a variety of ailments. Later, the activities of synthesized biogenic nanoparticles were also compared with various plant extracts aqueous, ethanolic and methanolic of t. The antibacterial activity of methanolic and ethyl acetate leaf extracts of tridax procumbens linn l. Antibacterial activity was investigated by disc and agarwell diffusion method. Evaluation of antimicrobial activity to investigate antimicrobial activity of tridax procumbens l.
In conclusion tridax procumbens leaf extract terminates most propitious source. The antibacterial activity of the plant parts extracts stem, root, leaf, flower and whole plant of tridax procumbens l. The antibacterial activity of tridax procumbens mediated fe3o4 nanoparticles was performed against pseudomonas aeruginosa using agar well diffusion method. Plant biotechnology lab 1, department of botany, university of rajasthan, jaipur302044, rajasthan, india. To investigate the phytochemical screening and antibacterial activity of pet. Anthocyanins are present in aqueous, methanolic, chloroform, alcoholic and ethyl acetate extracts of thespesia and totally absent in tridax. Green synthesis of silver nanoparticles using tridax. The flower plant extracts demonstrated good antimicrobial activity against these bacteria tested with inhibition zones. This study aimed at evaluating the phytochemical compounds and antibacterial activities of the leaf extract of tridax procumbens. The tridax procumbens flavonoids tpf, are well known for their medicinal properties among local natives. Mechanism of antibacterial action of the alcoholic extracts.
British journal of pharmaceutical research 14, 164173. Hence we can say the active components which are present cumbens are exbhiting antimicrobial activity from the above results. A plausible antibacterial green synthesized agnps from. It also proves the traditional claim with regard to. Antibacterial activity of tridax procumbens with special. Tanuja murab1, preeti chandurkar2, nidhi gujar3 and namita ahakey4 department of biotechnology, career college1,2,3,4, govindpura, bhopal m.
In this research the synthesis of silver nanoparticles using plant tridax procumbens has been investigated. Tridax procumbens plant has been used in traditional medicine to cure several ailments. Bharathi and others published antibacterial activity of tridax procumbens linn find, read and cite all the research. It has anticoagulant, antifungal, antidiarrhoel and insect repellent properties 11 12. Comparative study of antimicrobial activity of tridax procumbens and vernonia cinerea against bacterial strains of e. Ankita jain 1, dv rao 1, amla batra 1 and amita jain 2.
Bioactivities of extracts and isolation of compounds from. Phytochemical estimation and antioxidant activity were carried out for aqueous extract. Asian journal of research in biological and pharmaceutical. Pdf phytochemical and antibacterial studies of leaves of. The aim of the present study was to identify the bioactive compounds and to investigate the antibacterial activity from the leaves of. Tridax family compositae commonly known as ghamra and in english popularly called coat buttons because of appearance of flowers has been extensively used in ayurvadic system of medicine for various ailments and is. Phytochemicals and antibacterial activities of leaf. Phytochemical and antibacterial studies of leaves of. Scholars research library antibacterial potential of tridax. Antibacterial evaluation of aqueous and methanol extracts of tridax procumbens flower on selected gram negative and grampositive bacteria. Invitro antibacterial, antioxidant potentials and cytotoxic. On the basis of these activities of tridax procumbens.
Resistance emergency has necessitated continuous search for drugs with least toxicity, effective, affordable and ecofriendly which possess new mode of action to cater the rising emergency. The phytochemical analysis showed presence of tannins by cumbens linn and p. Foods free fulltext antihyperuricemia, antioxidant, and. Hence we can say the active components which are present t. Philomenas degree college, mysore, karnataka abstract. Biogenic synthesis of fe3o4 nanoparticles using tridax leaf. Plant pharmacological activity whole plant antimicrobial activity anticoagulant, hair tonic, antifungal and insect. Key words tridax procumbens, methonolic and ethyl acetate extract, antibacterial activity. Pdf antibacterial activity of tridax procumbens linn researchgate. Phytochemical estimation and antioxidant activity were carried out for aqueous.
The results lend credence to the folkloric use of this plant in treating microbial infection and showed that tridax procumbens leaves and stem could be exploited for new potent antimicrobial agents. The present work is concerned with exploring antimicrobial activity of. Fresh plant juice is applied twice a day for 34 days to cure cuts and wounds. The antimicrobial activity is greater in crude extracts than coloumn extracts table 2. Antimicrobial activity of methanolic extract of flowers of. Synthesized agnps were characterized by uv spectroscopy, ftiratr, sem, and tem analyses. Antioxidant, antidiabetic, antimicrobial and hemolytic. Himakshi bhatikushwaha and c p malik school of life sciences, jaipur national university, jaipur 302 017, india received 14 march 20. Phytochemical and pharmacological screening of combined. The mean of three replicates of zone of inhibition mm around well with tridax procumbens mediated. Phytochemicals and antibacterial activities of leaf extract.
It also proves the traditional claim with regard to tridax procumbens. Department of chemistry, college of natural sciences, can tho university. Chitra pai, ujjwala kulkarni, manjusha borderlands, sowmya murali p, mrudala yashwant deshmukh. In the present study, it was planned to investigate the phytochemical and in vitro antioxidant activity of the ethanol, methanol and aqueous extracts of tridax procumbens leaves. Shows that the antimicrobial activity of ag nanoparticles from tridax plant extract. Antimicrobial activity of aqueous extracts of two plants were investigated by agar disc welldiffusion method against bacterial pathogens staphylococcus aureus, bacillus subtilus, escherichia coli, k. Foods free fulltext antihyperuricemia, antioxidant. Flavonoids isolated from tridax procumbens tpf inhibit. Pseudomonas aeruginosa, while the petroleum ether extract did not shown antibacterial activity. Antibacterial activity of tridax procumbens with special reference to nosocomial pathogens. It also used in treating gastric problems, body pain, and rheumatic pains of joints.
Traditionally, the stem of tridax has been very much associated with antimicrobial activity mundada and shivhare, 2010. The leaf juice possesses antiseptic, insecticidal and parasiticidal properties and is used also to check haemorrhage from cuts, bruises and. January antimicrobial activity of tridax procumbens against. Previous researches have shown that the plant has antimicrobial activities and pharmacological effects. An aqueous extract was prepared similarly by using only the powder and 500 ml of sterile water. Sterols, tribulus terrestris, sida acuta, tridax procumbens introduction medicinal plants are essential part of human medicine, since the dawn of civilization and are the backbone of traditional medicine system in india 1. Asian journal of research in biological and pharmaceutical sciences. Antihyperuricemia, antioxidant, and antibacterial activities. Keeping this view in mind, the invitro antimicrobial activity of hot water and methanolic extracts of the leaves of tridax procumbens was tested by plate diffusion. Antibacterial activity of roots extracts from tridax. Phytochemical screening and in vitro antimicrobial activity of tridax procumbens l. Previous finding revealed antibacterial activity of t. Tridax procumbens linn is a tropically distributed medicinal plant. Antibacterial activity of different parts of tridax procumbens against.
Pdf antibacterial activity of tridax procumbens with. It has hepatoprotective activity, antiinflammatory 14, and. Synthesis of silver nanoparticles using tridax procumbens. Phytochemical and antibacterial studies of leaves of tridax. The results are represented in the figure 1, 2 and 3. Synthesis of silver nanoparticles using tridax procumbens and. The activity was done by agardisc and well diffusion method 16. Bioactivities of extracts and isolation of compounds from tridax procumbens l. The methanolic extract of tridax procumbens showed significant antimicrobial activity against positive staphylococcus aureus and on e. Pdf phytochemical screening and in vitro antimicrobial. R engineering college, sivakasi626140, tamil nadu abstract tridax procumbens, is a weed extensively used in traditional medicine as anticoagulant. Antimicrobial activity whole plant of tridax has reported for its antimicrobial activity on various species of bacteria. To the best of our knowledge, this is the first report on antibacterial activities of different phytochemical extracts and mode of action of the bacterial susceptibility compound from the leaves of t. Their antibacterial activities were evaluated in vitro against clinical bacterial isolates.
Invitro evaluation of the synergistic antimicrobial. The extract of whole plant of tridax showed antibacterial activity only against. Assessment of antibacterial and antifungal activities of. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. Jmphtr 2 2014 1622 issn 20531826 invitro evaluation of the synergistic antimicrobial activities of zingiber officinale rosc and tridax procumbens linn against selected pathogenic bacteria a. Antibacterial activity of agnps was evaluated against reference and mdr bacterial strains. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of. In the present study, it was planned to investigate the phytochemical and in vitro. This tridax procumbens plant was studied for its antimicrobial activity test by many researchers as alone 14. Antibacterial activity of tridaxprocumbens with special reference to nosocomial pathogens. The order of susceptibility of tridax procumbens on.
Perform the phytochemical screening of ethanolic, methanolic and chloroform extracts of leaves, stem and flower for tridax procumbens. India is gifted with varieties of large number of medicinal herbs because of variety of climatic conditions and seasons favorable for growth of many species of. A whole plant is squeezed between the palms of hands to obtain juice. The tpf are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. Pdf on jan 10, 2011, chitra pai and others published antibacterial activity of tridax procumbens with special reference to nosocomial. Plant part pharmacological activity reference whole plant antimicrobial activity against both grampositive and gramnegative bacteria r. A comparative antimicrobial analysis of tridax procumbens l. Scholars research library antibacterial potential of. The medicinal properties of different parts of tridax procumbens are listed in table 1 and phytochemical constituents of tridax procumbens plants are listed in table 2. Save nature to survive phytochemical screening and. The polyphenol and flavonoids are used for the treatment of various diseases triggered by oxidative stress. Anti microbial activity antimicrobial activity was explored by using acetone and methanolic extracts of plant parts as root, leaves and flowers respectively. The tpf have been reported to increase osteogenic functioning in mesenchymal stem cells. Pdf antimicrobial activity of methanolic extract of.
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